||Apricot PPV resistance trait-Badenes-2011
||In this study, we have constructed two high-density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of ‘Lito’ and ‘Goldrich’ PPV-resistant cultivars, respectively. Using these maps, and excluding genotype–phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in ‘Lito’ and ‘Goldrich’, respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30–40 cm encompassing PPVres. Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by highinformation content fingerprinting (HICF).
This project contains 2 QTL: